Electrochemiluminescence (ECL)

Electrochemiluminescence is a highly sensitive method for the detection of diverse analytes. Antibodies, peptides or proteins, cells, membranes or nucleic acids can be measured with the help of ECL. A large dynamic detection range of up to 5 logs enables the quantification of native levels of biomarkers. Applying ECL, femtomolar amounts of analytes are no longer a great challenge.

With the help of devices from Mesoscale Discovery (MSD^®), we are able to measure analytes by multiplexing in ultra-short time. MSD^® kits ready for use enable the analysis of up to 10 substances at the same time. High throughput analysis of standard biomarkers leads to time, cost and sample saving. Low sample volumes are advantageous especially in preclinics when the availability of serum is limited. Generally, 25 μl per well on the micro titer plate are absolutely sufficient.

ECL does not only enable the measurement in serum. Other matrices like blood, plasma or urine do not cause any problems. Homogeneous assay formates lead to stringent protocols in a GxP-compliant environment. If your assay is based on the application of another technology, don't worry: We can adapt it to the ECL platform without tremendous efforts. The cost for a conventional ELISA testing and a highly sensitive ECL measurement are really comparable at Celonic!

Some years ago, we have marketed ECL devices ourselves. Today we are no longer active in retail, but we apply ECL devices in the services we offer for our customers. Thus, our level of experience is really high. Are you interested in the detection of anti-drug-antibodies during a preclinical study? May we help you to detect biomarkers when conventional analytical techniques face their limit of detection? Please let us know, because we are the experts in ECL.